Have you ever heard of double beam UV spectrometer? It probably sounds like a big, fancy machine, but it is actually a really awesome tool that helps scientists find out about the world around us. Everyday, Labtech scientists depend on double beam UV spectrometers to facilitate their critical experiments and research. It makes it easier for them to learn about various substances and their properties by helping them collect crucial data such as this.
Let’s dissect how this machine operates. A double beam UV spectrometer is an absolute tool to determine the absorbance of compounds. When light passes through a sample, some of it gets absorbed by the molecules in that sample. By observing how much light is absorbed, scientists can (to varying degrees) figure out very important properties of the sample, like what it is composed of and how it behaves.
This is a double beam spectrometer, it has two beams of light. “One beam has directed on the sample of interest that scientists would like to study and the other beam is directed on a reference cell that has no sample in it. By comparing the two beams of light, the researchers can obtain a much sharper and more precise measurement of how much light the sample absorbs. It helps them draw better conclusions of their experiments.
Double beam UV spectrometry is employed in a wide variety of both scientific and industrial fields. For example, this tool is often used by medicine companies to verify that their drugs are safe and pure, making them able to be used by humans. This is extremely crucial, as this ensures the medicine has the proper active ingredients and no harmful chemicals. UV spectrometry can also be used by scientists to identify unknown substances. If a scientist finds a sample they don’t recognize, they can use a UV Spectrometer to learn more about what its properties might be and what it could possibly be.
This tool is also particularly useful for the study of samples that vary over time. For example, a standard UV spectrometer is often not able to yield accurate results when a chemist needs to know the amount of a substance that is being destroyed in a chemical reaction. But, with a double beam UV spectrometer, the scientists can measure over time and see how the sample varies. The information gathered this way is extremely useful for understanding (and sometimes even recreating in the lab) many biological and chemical processes.
Since a double beam UV spectrometer was a step-up from its predecessor, scientists kept improving the technology through the years. Automatic wavelength calibration, for example, is available on some modern spectrometers. This means that the system can account for any variations that occur by the light source in real time, so the measurements that the hardware collects are always correct and trusted.
Another improvement that is super exciting is the use of flow cells. Flow cells enable samples to be tested while passing through the spectrometer. This is particularly valuable for sectors like food and beverage testing, where speed and efficiency in sample analysis are paramount. Moreover, a few double beam UV spectrometers are equipped with software that allows for automating the data collection and analysis processes. This allows scientists to collect and interpret data even more quickly and easily.
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